Description:
Synthesis of peptide nucleic acids with modified nucleobases with high affinity to recognize and complex double-stranded RNA at physiologically relevant conditions
Background:
Compared to DNA, molecular recognition of double stranded RNA has received relatively little attention. Since it has been discovered that RNA can catalyze chemical reactions, the important role that non-coding RNAs play has grown. The ability to selectively recognize and control the function of such RNAs is highly useful for both fundamental research and practical applications.
Technology Overview:
The present invention provides a new monomer for preparation of peptide nucleic acid (PNA) carrying a novel 2-aminopyridine nucleobase. PNAs containing 2-aminopyridine and other modified nucleobases form stable and sequence selective triple helices with double-stranded RNA at physiologically relevant conditions. The modified PNAs display unique RNA selectivity by having two orders of magnitude higher affinity for the double-stranded RNAs than for the same DNA sequences. Results show that nucleobase-modified PNA bind and recognize complex biologically important RNAs, such as double helical precursors of microRNAs.
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Advantages:
- Ability to recognize double helical RNA in a sequence selective manner at physiologically relevant conditions.
- Strong and selective triple helical binding of peptide nucleic acids to double-stranded RNA.
Intellectual Property Summary: